AmScope, IN300TB-FL-MF143, Inverted Epi-fluorescence Trinocular Compound Microscope - 40X-800X / USB 3.0 / 1.4MP (Monochrome)
IN300TB-FL-MF143 Includes Only:
- One microscope body
- Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
- One pair 10X eyepieces
- One pair 20X eyepieces
- One phase-contrast annulus board
- One top-mount illuminator
- One rear-mount fluorescence illuminator
- One power supply for fluorescence illuminator
- One UV blocking shield
- One 30W halogen bulb
- One 100W mercury bulb
- One dust cover
- Color filters
- Two power cords
- One C-mount TEC USB camera
- One power adapter
- One AC power cord (North America plug)
- One USB 3.0 cable
- One software CD
- One heavy-duty case
Features:
- 40X to 800X expanded magnification range using a quadruple lens turret and additional 20X eyepieces for six unique settings.
- An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
- The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
- The infinity-corrected optical system ensures high-quality, high-resolution images.
- Specialized 10X and 20X objective lenses are included for phase-contrast applications.
- The trinocular photo port provides a dedicated interface for mounting a camera.
- 1.4MP ultra-sensative monochrome CCD camera for low-light applications with active cooling for reduced noise-floor
- Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.
The Viewing Head
The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.
Optics
The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 20X eyepieces expand the maximum magnification to 800X with 6 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.
The Stage
The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage's aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.
Transmitted Illumination
The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.
Phase Contrast
The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.
Epi-fluorescence Illumination
For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.
Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.
Digital Imaging
The ultra-sensitive 1.4MP monochrome CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive (2000mV/µm2) CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD's "global shutter" design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.
