IBI Scientific IB01015 5X RNA Loading Dye Kit

Features:

• Used in RNA electrophoresis when using a formaldehyde gel and MOPS buffer

The 5X RNA Loading Dye Kit contains reagents for denaturing and loading RNA samples onto a formaldehyde gel and using MOPS as a buffer. Reagents are nuclease-free and contain ultra-pure deionized formamide and DEPC water. To use, the RNA sample is dissolved in 10 µl of DEPC water and mixed with 35 µl of denaturing solution. Heat the sample to 65°C for 5 minutes. Once the solution has cooled, add 5 µl of loading dye. The sample is now ready to load into the agarose or formaldehyde gel.