IBI Scientific IB47113 Midi Fast-Ion Plasmid Kit, Endotoxin Free

IB47113 Includes Only:

• PM1 Buffer - 110mL
• PM2 Buffer - 110mL
• PM3 Buffer - 110mL
• PER Buffer - 40mL
• PEQ Buffer - 130mL
• PMC Buffer - 125mL and 260mL
• PEL Buffer - 220mL
• RNase A (50mg/ml) - 260 µm
• Plasmid Midi Column - 25 pcs
• I-Blue Lysis Buffer - 1.5mL

Features:

• The quality of the Midi Fast Ion Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 50 ml overnight E. coli (DH5α) culture, containing pBluescript (A600>2U/ ml).
• More than 120 µg of plasmid DNA is quantified with a spectrophotometer.
• The purified plasmid (1 µg) is used in EcoRI digestion, and checked by electrophoresis.

The Midi Fast Ion Plasmid Kit uses pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with a wash buffer. Finally, the purified DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than two hours. The purified plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.