IBI Scientific IB47231 Mini Genomic DNA Kit for 100 Preparations

IBI Scientific, IB47231, Mini Genomic DNA Kit for 100 Preparations, Plant/Fungi

IB47231 Includes Only:

• GP1 Buffer - 50mL
• GPX1 Buffer - 50mL
• GP2 Buffer - 15µL
• GP3 Buffer (Add Isopropanol) - 30mL (60mL)
• W1 Buffer - 45mL
• Wash Buffer (Add Ethanol) - 25mL (100mL)
• Elution Buffer - 30mL
• RNase - 550µL
• Filter Columns - 100
• GD Columns - 100
• 2 ml Collection Tubes - 200

Features:

• The quality of the Genomic DNA Mini Kit for Plant is tested on a lot-to-lot basis by isolating genomic DNA from 50mg young leaf samples.
• More than 10µg of genomic DNA is quantified with a spectrophotometer and checked by electrophoresis.

The Genomic DNA Mini Kit for Plants and Fungi provides a fast and economical method to isolate total DNA (genomic DNA, mitochondrial, and chloroplast) from plant tissue and cells. Samples are disrupted both by grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and filtered to remove cell debris and salt precipitates. In the presence of the binding buffer coupled with chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix of the spin column. The contaminants are washed with an ethanol-based wash buffer. The genomic DNA is then eluted by a low salt elution buffer or water. The protocol does not require DNA phenol extraction or alcohol precipitation. This entire procedure can be completed in 30 minutes or less and the purified genomic DNA is ready for PCR, Real-Time PCR, Southern Blotting, or RFLP.