IBI Scientific IB47432 gPure Extraction Kit, 1000mL

IB47432 Includes Only:

• Number of cells - 6 x 10⁹ mL
• Cell Lysis Buffer - 1000mL
• Protein Removal Buffer - 400mL
• DNA Hydration Buffer (10mM Tris-HCI, 1mM EDTA, pH 8.0) - 500mL
• RNase A (10 mg/mL) - 5mL

Features:

• gPure DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells.
• The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

The gPure DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial, or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution-based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands-on time. Initially, cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated and then rehydrated. The high-quality extracted DNA is ready for use in a variety of downstream applications.