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IBI's LB Agar Lennox is used in molecular genetic studies. It is a nutritionally rich media for the growth and maintenance of pure cultures of recombinant strains of E. coli. The inclusion of casein peptone and yeast extract supplies essential growth factors to the E. coli bacterial culture; such as nitrogen, carbon, sulfur, minerals, and vitamins. Sodium chloride provides essential electrolytes. Agar is used as a solidification agent. To prepare, mix 35 g of the medium in 1 L of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Then, distribute and autoclave at 121.0°C for 15 minutes. Store at room temperature, tightly sealed, and protect from moisture and light.
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