Sigma-Aldrich A2929-25G Agarose for Pulsed Field Running Gel, 25G

Sigma-Aldrich, A2929-25G, Agarose for Pulsed Field Electrophoresis Running Gel, 25G

Suitable for the separation of high molecular weight DNA. Gels are easy to handle and give faster separation and better resolution of high molecular weight DNA by field inversion electrophoresis than routine-use agarose.

Application

• Especially formulated for fast resolution of large DNA (10-2000 ·kb) by pulsed-field gel electrophoresis (PFGE) or conventional electrophoresis. Pulsed-field gel electrophoresis was developed in 1984 to deal with "reptation" of large DNA's whose size surpass the limit of resolution and all migrate at the same rate. Alternately pulsed, orthogonally oriented electric fields are applied across the gel. Every time the direction of the electric field changes, the large DNA's can no longer migrate until they become reoriented along the electric field. The time required for reorientation is dependent on the size of the DNA, therefore the DNA is fractionated by size.