IBI Scientific, IB53000B, MP-1015 Multi Purpose Horizontal Electrophoresis System, Starter Kit
IB53000B Includes Only:
- MP-1015 Electrophoresis System
- 5 ml of 6 X Loading Dye
- 1 L of 10 X TAE Buffer
- 1 L of 10 X TBE Buffer
- 100 g of Molecular Biology Grade Agarose
Features:
- IBI horizontal systems are constructed of 3/8" thick cast acrylic, the heaviest in the industry.
- No warping occurs when the system gets hot, and there are no crazy cracks in the plastic after time.
- The buffer tank on the IBI systems can be completely re-built, by the end-user, in minutes.
- IBI horizontal systems allow the user to cast gels Outside the buffer tank, this is much easier and allows the user to release a gel while a gel is running.
IBI's MP-1015 Multi-Purpose Gel Box (10 x 15 cm) is a general-purpose unit for both extended high-resolution linear runs as well as economical quick screens. It is equipped with recirculation ports for procedures requiring buffer recirculation. The MP-1015 IBI electrophoresis unit is constructed of durable cast acrylic, which will withstand high voltages and extreme temperatures without cracking or warping, unlike most molded units. The MP-1015 can be transformed into a quick screening unit with samples resolved in 30-60 minutes. Resolution of restriction digestions, plasmid purification, and ligation reactions are all possible with short runs. The MP-1015 is also compatible with a number of analytical and preparative combs for every horizontal electrophoresis application.
The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the MP-1015 is 140 V. In a 1% TBE gel this translates into a run time of approximately one hour. Lower voltages may be used and as a general rule, a 70 V run will take twice as long as a 140 V run. Higher voltages may be used to decrease run time. However, if the unit is being operated at higher voltages than 140 V the heat generated during electrophoresis may decrease sample resolution. Such artifacts may be avoided by running the unit in a cold room, recirculating buffer, or adding 1 X electrophoresis buffer "ice cubes" to keep the unit properly cooled. The suggested run parameters for the electrophoresis of RNA in agarose gels containing formaldehyde are 60-80 V.
