AmScope, IN480TB-FL-MF143C, IN480 Series Inverted Epi-Fluorescence Trinocular Compound Microscope 40X-1200X Magnification with 1.4MP Cooled Color CCD Camera
IN480TB-FL-MF143C Includes Only:
- One microscope
- One photo port
- One mechanical stage attachment
- One condenser
- Five brightfield objective lenses
- Three phase-contrast objective lenses
- One pair 10X eyepieces
- One pair 20X eyepieces
- One glass stage insert
- One metal stage insert
- Three specimen holders
- One phase-contrast annulus board
- One centering telescope
- Two bulbs
- Two power cables
- One dust cover
- One fluorescence lamp housing
- One power supply for fluorescence lamp
- One mercury HBO bulb
- One B1 filter block
- One G1 filter block
- One UV-blocking light shied
- One C-mount TEC USB camera
- One power adapter
- One AC power cord (North America plug)
- One USB 3.0 cable
- One heavy-duty case
Key Features:
- 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
- An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
- The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
- The infinity-corrected optical system ensures high-quality, high-resolution images.
- Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
- The trinocular photo port provides a dedicated interface for mounting a camera.
- 1.4MP ultra-sensative color CCD camera for low-light applications with active cooling for reduced noise-floor
- Professional software suite for Windows with advanced imaging functions and fluorescence tools. Lite version for Mac and linux
The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are comes for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.
The Viewing Head
The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.
Optics
The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.
The Stage
The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage's aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.
Koehler Transmitted Illumination
The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.
Epi-fluorescence Illumination
A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.
Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.
Digital Imaging
The ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software compatible for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.
