IBI Scientific, IB06015, Midi Select-D Microcentrifuge Spin Column, Sephadex G-50 in TE Buffer
IB06015 Includes Only:
- 50 spin columns
- 100 collection tubes
Features:
- DNase- and RNase-free
- Each DNase- and RNase-free column is prepackaged with Sephadex G-50 in sterile TE buffer.
- Two nuclease-free collection tubes (autoclavable) are supplied for each column.
- The optimal sample loading volume is 50µl with a maximum of 50µg of nucleic acid per column.
The Midi Select-D, G-50 microcentrifuge spin columns are intended for use in desalting, recovering DNA fragments (>72bp, smaller DNAs will be retained), and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from small volume 5' end-labeling reactions and fill-in labeling reactions utilizing a DNA polymerase. In addition, the Midi Select-D G-50 spin columns are RNase-free and can be used for the rapid purification of RNA (>72 bases) away from unincorporated ribonucleotides (rNTPs) and in other related applications. After brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume.
