Features:
- Certified nuclease-free and pyrogen-free
- 0.1 µm sterile-filtered into certified-clean polycarbonate bottles
- Steam-sterilized (autoclaved twice)
- 18.2 Megohm deionized
Applications:
- PCR
- RT-PCR
- Restriction enzyme assays
- Modifying enzyme assays
- Transfection
- Cloning
- Transformation
- All general molecular biology lab procedures
IBI Molecular Biology Grade Water is manufactured under stringent conditions. The purification process for this 18.2 megohm product includes continuous deionization, reverse osmosis, UV treatment, and a 0.1 µm filtration, followed by steam sterilization in the final packaging. The unique water purification process involves sterile filtration through a 0.1 µm filte, thus removing all bacteria, viruses, and pyrogens. Sterility is further maintained by sterile-filtering directly into an aseptic polycarbonate bottle (or container). These FDA-approved polycarbonate bottles are non-leaching and allow for IBI water products to be autoclaved in their final packaging, ensuring terminal sterilization. Unlike PET bottles, IBI polycarbonate bottles can be autoclaved multiple times. If compromised sterility of an open product is a concern, polycarbonate bottles allow for re-autoclaving to inactivate any contaminating nucleases. Re-autoclaving can be done on the liquid cycle at 121°C/15 psi. (Be sure to slightly disengage the lid during the autoclave cycle.)
The sterility of water is measured by the Sterility Assurance Level (SAL). IBI Molecular Biology Grade Water maintains the highest level of sterility with a SAL=10-6. Thus, the chance of a living organism being contained in the water is one in a million. In order to maintain sterility, only open this product in a sterile environment, i.e. clean room, tissue culture fume hood, etc. After manufacturing, water is tested for up to three days. Each lot is individually assayed to ensure that it meets the stated specifications prior to being released from quality control.
