IBI Scientific IB47030 Gel Extraction and PCR Cleanup Kit

Features:

• The IBI PCR Clean up and gel extraction kits are designed to recover or concentrate DNA fragments (50bp - 10kb) from agarose gel, PCR, or other enzymatic reactions.
• This method uses chaotropic salt to dissolve the agarose gel and denature the enzymes.
• The DNA fragments in the chaotropic salt are bound to the glass fiber matrix of the spin column.

Applications:

• PCR, Fluorescent or Radioactive Sequencing, Restrictive Digestion, DNA Labeling, and ligation.

The contaminants are washed with a wash buffer (containing ethanol) and the purified DNA fragments are eluted with a low salt elution buffer or pure water. Salts, enzymes, or unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are 90 - 95% for PCR clean-up. The entire procedure can be completed in 20 minutes, and the eluted DNA is ready for use in restriction digestion, ligation, PCR, fluorescent or radioactive sequencing, and DNA labeling.