IBI Scientific IB47153 2 x 96 Well Plasmid Kit

IB47153 Includes Only:

• P1 Buffer - 40mL
• P2 Buffer - 40mL
• P3 Buffer - 60mL
• Wash Buffer (Edd Ethanol) - 50mL (200mL)
• Elution Buffer - 30mL
• RNase A (50 mg/ml) - 130 µm
• Plasmid 96 Well Filter Plate - 2 pcs
• Plasmid 96 Well Binding Plate - 2 pcs
• 96 Deep Well Plate - 2 pcs
• 0.35 ml Collection Plate - 2 pcs
• Adhesive Film - 8 pcs

Features:

• 2x 96 Well Plasmid Kit
• Centrifuge or vacuum format
• 1 - 5 ml cultured bacterial cells
• Filter plates, deep well plates, and collection plates included

The 2 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 - 5 ml of cultured bacterial cells per/well. The kit utilizes a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. This kit requires a centrifuge or a vacuum manifold capable of running 96 well plates. IBI offers an IB47500 96 well vacuum manifold.

Typical yields are 20 - 35 µg for high-copy number plasmid and 3 - 10 µg for low-copy number plasmid from 4 ml of cultured bacterial cells (E. coli). The purified DNA is ready for use in restriction enzyme digestion, ligation, PCR and sequencing reactions.