IBI Scientific, IB47201, Mini Genomic DNA Kit for 25 Preparations, Blood and Cultured Cells
IB47201 Includes Only:
- RBC Lysis Buffer - 135mL
- GT Buffer - 30mL
- GB Buffer - 40mL
- W1 Buffer - 45 mL
- Wash Buffer (Add Ethanol) - 25 mL (100mL)
- Elution Buffer - 30mL
- GD Columns - 100
- 2 ml Collection Tubes - 200
Features:
- The quality of the Genomic DNA Mini Kits for Blood and Cultured Cells is tested on a lot-to-lot basis by isolating genomic DNA from 200 µl of whole human blood.
- The purified DNA (4-6 µg with an A260/A280 ratio of 1.6 - 1.8) is quantified with a spectrophotometer and checked by electrophoresis.
The Genomic DNA Mini Kits for Blood and Cultured Cells provide a fast and economical method for the purification of total DNA (including genomic, mitochondrial, and viral DNA) from fresh whole blood, plasma, serum, buffy coat, other bodily fluids, lymphocytes bacteria, and cultured cells. The method uses chaotropic salt to lyse cells and degrade proteins. The DNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. After the removal of contaminants, the purified DNA is eluted with a low salt elution buffer or water. The entire procedure can be completed in 40 minutes without the use of phenol extraction or alcohol precipitation.
