IBI Scientific IB47221 Mini Genomic DNA Kit for 50 Preparations, Tissue

IB47221 Includes Only:

• GT Buffer - 30mL
• GBT Buffer - 40mL
• W1 Buffer - 45mL
• Wash Buffer (Add Ethanol) - 25mL (100mL)
• Proteinase K (Add ddH₂O) - 11 mg (1.1mL)
• Elution Buffer - 30mL
• GS Columns - 50
• 2 ml Collection Tubes - 100
• Micropestle - 50

Features:

• The quality of the Genomic DNA Mini Kit for Tissue is tested on a lot-to-lot basis by isolating genomic DNA from a 20 mg mouse liver sample.
• The purified DNA (more than 10 µg with an A260/A280 ratio of 1.7 - 1.9) is quantified with a spectrophotometer and checked by electrophoresis.

The Genomic DNA Mini Kits for Tissue are specially designed for the purification of total DNA (including genomic mitochondrial and viral DNA) from a variety of animal tissues and cells. The provided micropestle can efficiently homogenize tissue samples to shorten the time in the lysis step. This method uses Proteinase K and chaotropic salt to lyse cells and degrade proteins. The DNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. After contaminants have been removed, the purified DNA is eluted with a low salt elution buffer or water. This entire procedure can be completed in under 60 minutes without phenol extraction or alcohol precipitation. The expected yield of genomic DNA is up to 50 µg and the purified DNA (with approximately 20-30 Kb) is suitable for PCR or other enzymatic reactions.