IBI Scientific IB47292 GBAC Mini Genomic DNA Kit, 300 Preparations

IB47292 Includes Only:

• Gram+ Buffer - 75 mL
• GT Buffer - 75 mL
• GB Buffer - 75 mL
• W1 Buffer - 130 mL
• Wash Buffer (Add Ethanol) - 50 ml (200 ml)
• Lysozyme - 250 mg
• Proteinase K (Add dd H2O) - 65 mg x 2 (6,5 ml)
• Elution Buffer - 75 mL
• Elution Buffer - 300
• 2 ml Collection Tubes - 600

Features:

• The gBAC Mini Genomic DNA Kit has been optimized for purifying total DNA (including genomic and viral DNA) from gram-negative and gram-positive bacterial cells.
• The provided gram-positive buffer, when combined with lysozyme, will efficiently lyse bacterial cell walls consisting of peptidoglycan.
• Chaotropic salt is then used to further lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column.
• Contaminants are removed using a wash buffer (containing ethanol) and the purified genomic DNA is eluted using a low salt elution buffer or TE.
• The entire procedure can be completed in one hour without phenol-chloroform extraction or alcohol precipitation.
• The purified DNA is suitable for PCR and other enzymatic reactions.

The quality of the gBAC Mini DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from E. coli (1x10⁹) culture (OD600=1.3, 1 ml) harvested by centrifugation at 16,000 xg for one minute. 10 µl from a 50 µl eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.