IBI Scientific IB47322 Mini Total RNA Kit for 100 Preparations

IBI Scientific, IB47322, Mini Total RNA Kit for 100 Preparations, Blood and Cultured Cells

IB47322 Includes Only:

• RBC Lysis Buffer - 200mL
• RB Buffer - 60mL
• DNase I (20U/µL) - 550µL
• DNase I Reaction Buffer - 5mL
• W1 Buffer - 50mL
• Wash Buffer (Add Ethanol) - 25mL (100mL) + 12.5mL (50mL)
• RNase-free water - 15mL
• RB Columns - 100
• 2mL Collection tubes - 200

Features:

• The quality of the Total RNA mini Kit for blood and cultured cells is tested on a lot-to-lot basis by isolating total RNA from blood and cultured cell samples.
• The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

The Mini Total RNA Sample Kit for blood and cultured cells is specially designed for the purification of total RNA from fresh whole human blood and cultured cells. This method uses detergents and chaotropic salt to lyse cells and inactivate RNase. The RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed following the wash step, the purified RNA is eluted by RNase-Free water. The entire procedure can be completed within 30 minutes. The purified RNA is ready for RT-PCR, Northern Blotting, Primer Extension, and cDNA library construction.