IBI Scientific IB47701 DNA/RNA/Protein Extract, 50 Preparations

IB47701 Includes Only:

• RBC Lysis Buffer - 100 mL
• DR Buffer - 30 ml
• RW1 Buffer - 30 ml
• RPE Buffer (Add Ethanol) - 25 ml (100 ml)
• W1 Buffer - 45 ml
• Wash Buffer (Add Ethanol) - 12.5 ml (50 ml)
• RNase-free Water - 6 ml
• Elution Buffer - 30 ml
• DV Buffer (8M Urea) - 6 ml
• Protein Loading Dye - 2 ml
• RB Columns - 50
• 2 ml Collection Tubes - 200

Features:

• The DNA RNA Protein isolation Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.
• Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column.
• The flow-through can then be transferred to the RNA Spin column for RNA binding.
• Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water.
• DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes.
• The purified DNA, with approximately 20 - 30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis.
• The purified proteins can be directly analyzed on an SDS-PAGE and subsequent western blot.

The quality of the DNA RNA Protein isolation Kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1x10⁶ cultured animal cells. The purified DNA and total RNA are quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. The purified protein is quantified by the Bradford assay analyzed on SDS-PAGE.