IBI Scientific IB72003 TE Buffer, 500mL

Features:

• Sterile
• Endotoxin Free
• pH - 8.0
• This scrutiny in manufacturing provides the assurance to each customer that their precious nucleic acids (DNA and RNA) are safe from any degradation.
• IBI TE buffer is packaged in medical grade non-leaching polycarbonate bottles to ensure purity levels and sterility are maintained.
• This TE Buffer is certified nuclease-free and endotoxin free by way of LAL testing to < 0.05 EU/ml.
• This TE buffer has a pH of 8.0 if your lab/process requires a different pH or an entirely different specialized buffer.

Tris EDTA, commonly known as TE Buffer, is synonymous with the storage and solubilization of DNA, cDNA, and RNA. Though primarily a pH buffer, it is also a protective agent for the purified nucleic acids due to the presence of the chelating agent EDTA, which inactivates the nucleases. A pH of 7.5 is recommended for RNA and a pH of 8 is most commonly used for DNA. Based on historic data the nucleases are less effective at a pH higher than 7.5 however, steam sterilization and ultra-filtration (0.01 micron) can further eliminate possibilities of remnant nucleases. The certified nuclease-free buffers are prepared and tested for nuclease activity. Each buffer is prepared using double distilled water, which is free of all divalent ions that tend to activate any nucleases, and is terminally sterilized using autoclavable packaging. Each bottle of TE buffer is steam sterilized which ensures heat inactivation and denaturation of all building blocks of life. The combination of ultra-filtration and terminal steam sterilization makes product truly unique. Furthermore, IBI Scientific is the only manufacturer to provide a certified nuclease-free and endotoxin-free TE buffer.