IBI Scientific IB74050 Methylene Blue, 25 gm

Features:

• Used to stain agarose gels when recovery of DNA is required
• Cannot be shipped to a personal residence.

Methylene Blue is a recommended stain for agarose gels when recovery of DNA is required. The benefit of using methylene blue (rather than ethidium bromide) is the ability to use a white lightbox for visualization and photography in place of a UV lightbox. Using a white light box eliminates mutations that may affect the integrity of the isolated DNA. To stain DNA, stain a 1% gel for 15 min with 0.02% methylene blue solution in distilled water. Destain with distilled water for 15 minutes. To stain RNA bonded to nylon or nitrocellulose membranes, soak the dried membrane in 5% acetic acid at room temperature for 15 minutes. Then, place the filter in 0.5 M sodium acetate (pH 5.2) and 0.04% methylene blue for 5 - 10 minutes at room temperature. To destain, rinse the filter in water for 5 - 10 minutes.