Sigma-Aldrich 93443-500ML Triton X-100 Solution, BioUltra, 500ML

Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

• Triton-X has been used:
• for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
• As a component of lysis buffer used for homogenization of cells
• As a component of blocking buffer in immunofluorescence staining
• For the preparation of cells in double fluorescence staining

Biochem/physiol Actions

• Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.