Sigma-Aldrich H4034-500G HEPES, BioPerformance Certified, 500G

At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.

Application

• HEPES has been used:
• To supplement Dulbecco's modified Eagle's medium to culture and maintain cell lines
• As a component of platelet suspension buffer
• To supplement Hank's basic salt solution, which is used to wash pancreatic tissue
• As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
• For the adjustment and maintenance of pH of biological solutions
• As a component of Hank's balanced salt solution (HBSS) and dissociation medium to study neuronal development
• For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
• As a component of keratinocyte and fibroblast culture medium