Sigma-Aldrich S5761-500G Sodium Bicarbonate, 500G

Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.

Application

• Sodium bicarbonate has been used:
• as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)
• as a component of a 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's nutrient mixture F-12 during the culture of bovine microvascular endothelial cells
• as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells
• as a component of medium used for cell culture of human embryonic kidney cells
• as a component of Beltsville thawing solution used for the dilution of semen