Sigma-Aldrich T6066-1KG Trizma Base, BioPerformance Certified, 1KG

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

• Trizma has been used:
• As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
• As a component of pre-hybridization buffer in in situ hybridization
• To suspend dried protein before SDS-PAGE protein characterization
• As a component of lysis buffer to wash and lyse cells before immunoprecipitation
• In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
• In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
• In capillary electrochromatography and UV analysis of tocopherols and tocotrienols